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EntericBio Pathogen Detection Process

EntericBio realtime test has been designed by Medical Scientists - for Medical Scientists. The goal was to make the test as simple as possible, 'error proofing' at every stage.

The EntericBio realtime pathogen detection process

These assays work directly from the faecal / stool sample and do not require nucleic acid extraction/purification. The entire mastermix required to perform each test is lyophilised into individual reaction wells. Each reaction well contains an internal amplification control to monitor for PCR inhibition. A positive amplification control is provided with each kit.

 

A typical run of 48 samples has a turnaround time to result of less than 3 hours with typically 30 mins hands-on time.

The Faecal / Stool test requires

 

  • NO DNA extraction
  • NO reagent preparation
  • NO manual pipetting
  • NO enrichment

Truly Revolutionary

Process Summary

Step 1: Resuspend sample and release DNA
A swab is lightly coated with the stool sample, the swab is re-suspended in a tube of EntericBio Faecal / Stool Preparation Solution (SPS) then heated on an EntericBio heat station at 103⁰C for 30 min.

Process Summary Step 1
Process Summary Step 2

Step 2: Automated transfer of sample into PCR well
The heat treated samples are placed on the EntericBio workstation for fully automated transfer of the processed samples directly to the lyophilised reaction wells using the EntericBio programme.

Process Summary Step 2

Step 3: Automated pathogen detection
The wells are capped and transferred to realtime PCR instrument for automated amplification, detection and analysis with the EntericBio programme.

Process Summary Step 3