MULTIPLEX IMMUNOHISTOCHEMISTRY ANTIBODIES

PRAME (preferentially expressed antigen in melanoma) is located on chromosome 22q11.22 and encodes a 509 amino acid protein. PRAME is an autosomal cancer-testis antigen (CTA) gene. PRAME is expressed in melanoma, various nonmelanocytic malignant neoplasms, including nonsmall cell lung cancer, breast carcinoma, renal cell carcinoma, ovarian carcinoma, leukemia, synovial sarcoma, and myxoid liposarcoma. Normal healthy tissues are not known to express PRAME except for testis, ovary, placenta, adrenals, and endometrium.

The mouse monoclonal antibody p40 [BC28] recognizes an epitope unique to the p40 protein and may have applications in cases where p63 has traditionally been used.

S100 recognizes proteins of 21-24 kDa, identified as the A and B subunits of S100 protein. S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C.

Uroplakin II is a 15 kDa protein component of urothelial plaques. Studies have shown Uroplakin II mRNA was highly specific and was expressed in both bladder cancer tissues and peripheral blood of patients with primary and metastatic urothelial carcinoma of the bladder.

IgG4 is an immunoglobulin G subclass of antibody. Mouse monoclonal IgG4 [HP6025] is specific for the Fc region of human IgG4. IgG4 can be helpful in the diagnosis of IgG4 related systemic disease (IgG4-RSD).

H3K27me3 (histone 3 lysine 27 trimethylation) is an epigenetic mark that plays a critical role in regulation of gene expression. The dysregulation of H3K27me3 is implicated in the genesis and progression of cancer.

Neurotrophic tyrosine receptor kinase (NTRK) proto-oncogenes NTRK1, NTRK2, and NTRK3 (that encode TRK A, TRK B, and TRK C proteins, respectively) may form gene fusions through their kinase domains, driving tumor development.

p63, a homolog of the tumor suppressor p53, has been identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate.