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EntericBio Pathogen Detection Process

EntericBio realtime test has been designed by laboratorians - for laboratorians with the goal to make the test as simple as possible, 'error proofing' at every stage.

The EntericBio realtime pathogen detection process

These assays work directly from the stool sample received in Cary-Blair medium and does not require nucleic acid extraction/purification. The entire mastermix required to perform each test is lyophilized into individual reaction wells. Each reaction well contains an internal amplification control to monitor for PCR inhibition. A positive amplification control is provided with each kit.

 

*Not available for sale in the US

**Product in development. Specifications subject to change.

 

A typical run of 32 samples has a turnaround time to result of less than 3 hours with typically 30 mins hands-on time.

The Fecal / Stool test requires

 

  • NO DNA extraction
  • NO reagent preparation
  • NO manual pipetting
  • NO enrichment

Truly Revolutionary

Process Summary

Step 1: Resuspend sample and release DNA
A flocked swab is lightly coated with the stool sample, received in Cary-Blair medium, the stool is re-suspended in a tube of EntericBio Fecal / Stool Preparation Solution (SPS) then heated on an EntericBio heat station at 103⁰C (217.4 Fahrenheit) for 30 min.

Process Summary Step 1
Process Summary Step 2

Step 2: Automated transfer of sample into PCR well
The heat treated samples are placed on the EntericBio workstation for fully automated transfer of the processed samples directly to the lyophilized reaction wells using the EntericBio program.

Process Summary Step 2

Step 3: Automated pathogen detection
The wells are capped and transferred to realtime PCR instrument for automated amplification, detection and analysis with the EntericBio program.

Process Summary Step 3